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Article

Maltose gradient-induced biosensor-based high-throughput screening for directed evolution of maltogenic amylase from Bacillus stearothermophilus.

International journal of biological macromolecules - 1 Nov 2024

Wang Jiayuan, Han Laichuang, Teng Maofang, Li Qinghua, Zhou Jingwen, Li Jianghua, Du Guocheng, Zhang Guoqiang

Abstract excerpt

Maltogenic amylase is a starch-hydrolyzing enzyme commonly used in bread baking and high-concentration maltose syrup production. However, low catalytic activity limits its industrial application. Improving catalytic activity based on molecular modification and directed evolution requires a High-Throughput Screening (HTS) method. In this study, a maltose gradient-induced (MaGI) biosensor was designed and applied...

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Identifiers

PMID: 39419146
DOI: 10.1016/j.ijbiomac.2024.136586

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