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Article

High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries.

Journal of biotechnology - 10 Jun 2017

Strezoska Žaklina, Perkett Matthew R, Chou Eldon T, Maksimova Elena, Anderson Emily M, McClelland Shawn, Kelley Melissa L, Vermeulen Annaleen, Smith Anja van Brabant

Abstract excerpt

The CRISPR-Cas9 system has been utilized for large-scale, loss-of-function screens mainly using lentiviral pooled formats and cell-survival phenotypic assays. Screening in an arrayed format expands the types of phenotypic readouts that can be used to now include high-content, morphology-based assays, and with the recent availability of synthetic crRNA libraries, new studies are emerging. Here, we use a cell cycle...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28445693
DOI: 10.1016/j.jbiotec.2017.04.017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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