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Article

A strategy for L-isoleucine dioxygenase screening and 4-hydroxyisoleucine production by resting cells.

Bioengineered - 1 Jan 2018

Zhang Chenglin, Ma Jie, Li Zhixiang, Liang Yunlong, Xu Qingyang, Xie Xixian, Chen Ning

Abstract excerpt

L-Isoleucine dioxygenase (IDO) specifically converts L-isoleucine(L-Ile) to 4-hydroxyisoleucine(4-HIL). To obtain IDO with improved activity, a strategy was developed that is dependent on the restoration of succinate-minus E. coli cell growth by the coupling of L-Ile hydroxylation and the oxidation of α-ketoglutarate(α-KGA) to succinate. Five mutants were obtained with this strategy, and the characteristics of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28430004
DOI: 10.1080/21655979.2017.1304872
PMCID: PMC5972919

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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