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Article

RNA-guided Cas9 as an in vivo desired-target mutator in maize.

Plant biotechnology journal - 1 Dec 2017

Li Chuxi, Liu Changlin, Qi Xiantao, Wu Yongchun, Fei Xiaohong, Mao Long, Cheng Beijiu, Li Xinhai, Xie Chuanxiao

Abstract excerpt

The RNA-guided Cas9 system is a versatile tool for genome editing. Here, we established a RNA-guided endonuclease (RGEN) system as an in vivo desired-target mutator (DTM) in maize to reduce the linkage drag during breeding procedure, using the LIGULELESS1 (LG1) locus as a proof-of-concept. Our system showed 51.5%-91.2% mutation frequency in T0 transgenic plants. We then crossed the T1 plants stably expressing DTM...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28379609
DOI: 10.1111/pbi.12739
PMCID: PMC5698053

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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