Article
An Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize.
Plant biotechnology journal - 1 Feb 2017
Char Si Nian, Neelakandan Anjanasree K, Nahampun Hartinio, Frame Bronwyn, Main Marcy, Spalding Martin H, Becraft Philip W, Meyers Blake C, Walbot Virginia, Wang Kan, Yang Bing
Abstract excerpt
CRISPR/Cas9 is a powerful genome editing tool in many organisms, including a number of monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler compared to other nuclease-based genome editing tools, optimization requires the consideration of the DNA delivery and tissue regeneration methods for a particular species to achieve accuracy and efficiency. Here, we describe a public sector...
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