Article
High-efficiency genome editing using a dmc1 promoter-controlled CRISPR/Cas9 system in maize.
Plant biotechnology journal - 1 Nov 2018
Feng Chao, Su Handong, Bai Han, Wang Rui, Liu Yalin, Guo Xianrui, Liu Chang, Zhang Jing, Yuan Jing, Birchler James A, Han Fangpu
Abstract excerpt
Previous studies revealed that the promoters for driving both Cas9 and sgRNAs are quite important for efficient genome editing by CRISPR/Cas9 in plants. Here, we report our results of targeted genome editing using the maize dmc1 gene promoter combined with the U3 promoter for Cas9 and sgRNA, respectively. Three loci in the maize genome were selected for targeting. The T0 plants regenerated were highly efficiently...
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