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Article

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA.

Plant physiology - 1 Oct 2015

Svitashev Sergei, Young Joshua K, Schwartz Christine, Gao Huirong, Falco S Carl, Cigan A Mark

Abstract excerpt

Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific insertion of a trait gene using clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)-guide RNA technology are reported in maize. DNA vectors expressing maize codon-optimized Streptococcus pyogenes Cas9 endonuclease and single guide RNAs were cointroduced with or without DNA repair templates into...

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Source

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Identifiers

PMID: 26269544
DOI: 10.1104/pp.15.00793
PMCID: PMC4587463

Genes

LIG1
ALS2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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