Article
Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system.
Journal of genetics and genomics = Yi chuan xue bao - 20 Feb 2014
Liang Zhen, Zhang Kang, Chen Kunling, Gao Caixia
Abstract excerpt
Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, for the first time, targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. We designed five TALENs targeting 4 genes, namely ZmPDS, ZmIPK1A,...
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