Article
PDZ domain-dependent regulation of NHE3 protein by both internal Class II and C-terminal Class I PDZ-binding motifs.
The Journal of biological chemistry - 19 May 2017
Cha Boyoung, Yang Jianbo, Singh Varsha, Zachos Nicholas C, Sarker Rafiquel I, Chen Tian-E, Chakraborty Molee, Tse Chung-Ming, Donowitz Mark
Abstract excerpt
NHE3 directly binds Na+/H+ exchanger regulatory factor (NHERF) family scaffolding proteins that are required for many aspects of NHE3 regulation. The NHERFs bind both to an internal region (amino acids 586-660) of the NHE3 C terminus and to the NHE3 C-terminal four amino acids. The internal NHERF-binding region contains both putative Class I (-592SAV-) and Class II (-595CLDM-) PDZ-binding motifs (PBMs). Point...
Topics
- Amino Acid Motifs
- Calcium
- Cell Line
- Cell Membrane
- Cyclic GMP
- Humans
- Mutation
- PDZ Domains
- Phosphoproteins
- Protein Binding
- Protein Stability