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Article

A Gradient of Sitewise Diversity Promotes Evolutionary Fitness for Binder Discovery in a Three-Helix Bundle Protein Scaffold.

Biochemistry - 21 Mar 2017

Woldring Daniel R, Holec Patrick V, Stern Lawrence A, Du Yang, Hackel Benjamin J

Abstract excerpt

Engineered proteins provide clinically and industrially impactful molecules and utility within fundamental research, yet inefficiencies in discovering lead variants with new desired functionality, while maintaining stability, hinder progress. Improved function, which can result from a few strategic mutations, is fundamentally separate from discovering novel function, which often requires large leaps in sequence...

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Identifiers

PMID: 28248518
DOI: 10.1021/acs.biochem.6b01142
PMCID: PMC5521020

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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