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Article

Deep mutational scanning reveals a de novo disulfide bond and combinatorial mutations for engineering thermostable myoglobin.

Protein science : a publication of the Protein Society - 1 May 2025

Küng Christoph, Protsenko Olena, Vanella Rosario, Nash Michael A

Abstract excerpt

Engineering protein stability is a critical challenge in biotechnology. Here, we used massively parallel deep mutational scanning (DMS) to comprehensively explore the mutational stability landscape of human myoglobin (hMb) and identify key mutations that enhance stability. Our DMS approach involved screening over 10,000 hMb variants by yeast surface display, single-cell sorting, and high-throughput DNA...

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PMID: 40247745
DOI: 10.1002/pro.70112
PMCID: PMC12006728

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