Article
Innovative metabolic pathway design for efficient l-glutamate production by suppressing CO2 emission.
Journal of bioscience and bioengineering - 1 Mar 2007
Chinen Akito, Kozlov Yuri I, Hara Yoshihiko, Izui Hiroshi, Yasueda Hisashi
Abstract excerpt
In the pathway of L-glutamic acid (L-Glu) biosynthesis in Corynebacterium glutamicum, 1 mol of L-Glu is synthesized from 1 mol of glucose at a cost of 1 mol of carbon dioxide (CO(2)), with a maximum theoretical yield of 81.7% by weight. We have designed an innovative pathway for efficient L-Glu production employing phosphoketolase (PKT) to bypass the CO(2)-releasing pyruvate dehydrogenase reaction, thereby...
Topics
- Aldehyde-Lyases
- Base Sequence
- Bifidobacterium
- Biomedical Engineering
- Carbon Dioxide
- Cloning, Molecular
- Corynebacterium glutamicum
- DNA, Bacterial
- Genes, Bacterial
- Glutamic Acid
- Models, Biological