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Biochemical characterization and mutational analysis of a novel flap endonuclease 1 from Thermococcus barophilus Ch5.

The international journal of biochemistry & cell biology - 1 Feb 2022

Lin Tan, Zhang Likui, Jiang Donghao, Wu Leilei, Chen Kaige, Li Li, Jin Cuili, Li Zheng, Oger Philippe

Abstract excerpt

Flap endonuclease 1 (FEN1) plays important roles in DNA replication, repair and recombination. Herein, we report biochemical characteristics and catalytic mechanism of a novel FEN1 from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5 (Tb-FEN1). As expected, the recombinant Tb-FEN1 can cleave 5'-flap DNA. However, the enzyme has no activity on cleaving pseudo Y DNA, which sharply contrasts with...

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PMID: 34990837
DOI: 10.1016/j.biocel.2021.106154

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