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Article

CRISPR/Cpf1-mediated DNA-free plant genome editing.

Nature communications - 16 Feb 2017

Kim Hyeran, Kim Sang-Tae, Ryu Jahee, Kang Beum-Chang, Kim Jin-Soo, Kim Sang-Gyu

Abstract excerpt

Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of recombinant Cpf1 proteins with in vitro transcribed or chemically synthesized target-specific crRNAs into...

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Identifiers

PMID: 28205546
DOI: 10.1038/ncomms14406
PMCID: PMC5316869

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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