Article
Efficient disruption and replacement of an effector gene in the oomycete Phytophthora sojae using CRISPR/Cas9.
Molecular plant pathology - 1 Jan 2016
Fang Yufeng, Tyler Brett M
Abstract excerpt
Phytophthora sojae is an oomycete pathogen of soybean. As a result of its economic importance, P. sojae has become a model for the study of oomycete genetics, physiology and pathology. The lack of efficient techniques for targeted mutagenesis and gene replacement have long hampered genetic studies of pathogenicity in Phytophthora species. Here, we describe a CRISPR/Cas9 system enabling rapid and efficient genome...
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