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Article

Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS.

Analytical chemistry - 6 Oct 2020

Brower Kara K, Khariton Margarita, Suzuki Peter H, Still Chris, Kim Gaeun, Calhoun Suzanne G K, Qi Lei S, Wang Bo, Fordyce Polly M

Abstract excerpt

In the past five years, droplet microfluidic techniques have unlocked new opportunities for the high-throughput genome-wide analysis of single cells, transforming our understanding of cellular diversity and function. However, the field lacks an accessible method to screen and sort droplets based on cellular phenotype upstream of genetic analysis, particularly for large and complex cells. To meet this need, we...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32900183
DOI: 10.1021/acs.analchem.0c02499
PMCID: PMC7670281

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