Targeted mutagenesis of a specific gene in yeast.

Abstract excerpt

Mutational analysis is a powerful experimental method to probe gene function. Gene deletions and mutations conferring loss of function or conditional lethality indicate if a gene is essential or not under a variety of experimental conditions. Point mutations can reveal information about function that is not possible from studies of the wild-type gene in vivo or the purified gene product in vitro. Here, we...

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