Article
Prion Transfection of Yeast.
Cold Spring Harbor protocols - 1 Feb 2017
Edskes Herman K, Kryndushkin Dmitry, Shewmaker Frank, Wickner Reed B
Abstract excerpt
Transfection of yeast with amyloid filaments, made from recombinant protein or prepared from extracts of cells infected with a prion, has become an important method in characterizing yeast prions. Here, we describe a method for transmission of [URE3] with Ure2p amyloid that is based on a previously published protocol for transfection with Sup35p filaments to make cells [PSI+]. This method may be used for other...
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