Article
Study of Amyloids Using Yeast.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Wickner Reed B, Kryndushkin Dmitry, Shewmaker Frank, McGlinchey Ryan, Edskes Herman K
Abstract excerpt
We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
