Article
The role of local residue environmental changes in thermostable mutants of the GH11 xylanase from Bacillus subtilis.
International journal of biological macromolecules - 1 Apr 2017
Silva Sérgio Bergamachi, Pinheiro Matheus Pinto, Fuzo Carlos Alessandro, Silva Samuel Reghim, Ferreira Tatiane Lopes, Lourenzoni Marcos Roberto, Nonato M Cristina, Vieira Davi Serradella, Ward Richard John
Abstract excerpt
A thermostable variant of the mesophilic xylanase A from Bacillus subtilis (BsXynA-G3_4x) contains the four mutations Gln7His, Gly13Arg, Ser22Pro, and Ser179Cys. The crystal structure of the BsXynA-G3_4x has been solved, and the local environments around each of these positions investigated by molecular dynamics (MD) simulations at 328K and 348K. The structural and MD simulation results were correlated with...
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