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Article

In vivo assay for low-activity mutant forms of Escherichia coli ribonucleotide reductase.

Journal of bacteriology - 1 Feb 2003

Ekberg Monica, Birgander Pernilla, Sjöberg Britt-Marie

Abstract excerpt

Ribonucleotide reductase (RNR) catalyzes the essential production of deoxyribonucleotides in all living cells. In this study we have established a sensitive in vivo assay to study the activity of RNR in aerobic Escherichia coli cells. The method is based on the complementation of a chromosomally encoded nonfunctional RNR with plasmid-encoded RNR. This assay can be used to determine in vivo activity of RNR mutants...

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Identifiers

PMID: 12562785
DOI: 10.1128/JB.185.4.1167-1173.2003
PMCID: PMC142847

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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