Article
Inactivation of ribonucleotide reductase by (E)-2'-fluoromethylene-2'-deoxycytidine 5'-diphosphate: a paradigm for nucleotide mechanism-based inhibitors.
Biochemistry - 25 Jun 1996
van der Donk W A, Yu G, Silva D J, Stubbe J, McCarthy J R, Jarvi E T, Matthews D P, Resvick R J, Wagner E
Abstract excerpt
Ribonucleotide reductase (RDPR) from Escherichia coli catalyzes the conversion of nucleotides to deoxynucleotides and is composed of two homodimeric subunits: R1 and R2. (E)- and (Z)-2'-fluoromethylene-2'-deoxycytidine 5'-diphosphate (FMCDP) are time dependent inactivators of this protein, with a...
Topics
- Alkylation
- Animals
- Antineoplastic Agents
- Base Sequence
- Deoxycytosine Nucleotides
- Electron Spin Resonance Spectroscopy
- Enzyme Inhibitors
- Escherichia coli
- Isomerism
- Kinetics
- Mice
- Models, Chemical
- Molecular Sequence Data
- Mutation