Article
Electron injection through a specific pathway determines the outcome of oxygen activation at the diiron cluster in the F208Y mutant of Escherichia coli ribonucleotide reductase protein R2.
Biochemistry - 27 Jan 1998
Parkin S E, Chen S, Ley B A, Mangravite L, Edmondson D E, Huynh B H, Bollinger J M
Abstract excerpt
Protein R2 of ribonucleotide reductase from Escherichia coli contains a dinuclear iron cluster, which reductively activates O2 to produce the enzyme's functionally essential tyrosyl radical by one-electron oxidation of residue Y122. A key step in this reaction is the rapid injection of a single e...
Topics
- Ascorbic Acid
- Catechols
- Electron Spin Resonance Spectroscopy
- Electron Transport
- Escherichia coli
- Iron
- Metalloproteins
- Mutation
- Oxygen
- Phenylalanine
- Recombinant Proteins
- Ribonucleotide Reductases
- Spectrophotometry
- Spectroscopy, Mossbauer