Article
Gated Proton Release during Radical Transfer at the Subunit Interface of Ribonucleotide Reductase.
Journal of the American Chemical Society - 13 Jan 2021
Cui Chang, Greene Brandon L, Kang Gyunghoon, Drennan Catherine L, Stubbe JoAnne, Nocera Daniel G
Abstract excerpt
The class Ia ribonucleotide reductase of Escherichia coli requires strict regulation of long-range radical transfer between two subunits, α and β, through a series of redox-active amino acids (Y122•[β] ↔ W48?[β] ↔ Y356[β] ↔ Y731[α] ↔ Y730[α] ↔ C439[α]). Nowhere is this more precarious than at the subunit interface. Here, we show that the oxidation of Y356 is regulated by proton release involving a specific...
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