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Article

Structural and functional characterization of two mutated R2 proteins of Escherichia coli ribonucleotide reductase.

European journal of biochemistry - 1 Apr 1996

Larsson A, Climent I, Nordlund P, Sahlin M, Sjöberg B M

Abstract excerpt

The R2 protein of ribonucleotide reductase from Escherichia coli is a homodimeric tyrosyl-radical-containing enzyme with two identical dinuclear iron centers. Two randomly generated genomic mutants, nrdB-1 and nrdB-2, that produce R2 enzymes with low enzymatic activity, have been cloned and characterized to identify functionally important residues and areas of the enzyme. The mutations were identified as Pro348...

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PMID: 8620893
DOI: 10.1111/j.1432-1033.1996.0058n.x

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