Article
Ultrasensitive single-genome sequencing: accurate, targeted, next generation sequencing of HIV-1 RNA.
Retrovirology - 20 Dec 2016
Boltz Valerie F, Rausch Jason, Shao Wei, Hattori Junko, Luke Brian, Maldarelli Frank, Mellors John W, Kearney Mary F, Coffin John M
Abstract excerpt
BACKGROUND: Although next generation sequencing (NGS) offers the potential for studying virus populations in unprecedented depth, PCR error, amplification bias and recombination during library construction have limited its use to population sequencing and measurements of unlinked allele frequencies. Here we report a method, termed ultrasensitive Single-Genome Sequencing (uSGS), for NGS library construction and...
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