Article
Accurate sampling and deep sequencing of the HIV-1 protease gene using a Primer ID.
Proceedings of the National Academy of Sciences of the United States of America - 13 Dec 2011
Jabara Cassandra B, Jones Corbin D, Roach Jeffrey, Anderson Jeffrey A, Swanstrom Ronald
Abstract excerpt
Viruses can create complex genetic populations within a host, and deep sequencing technologies allow extensive sampling of these populations. Limitations of these technologies, however, potentially bias this sampling, particularly when a PCR step precedes the sequencing protocol. Typically, an unknown number of templates are used in initiating the PCR amplification, and this can lead to unrecognized sequence...
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