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Analyzing the miRNA content of extracellular vesicles by fluorescence nanoparticle tracking.

Nanomedicine : nanotechnology, biology, and medicine - 1 Feb 2017

Baldwin Scott, Deighan Clayton, Bandeira Elga, Kwak Kwang J, Rahman Mohammad, Nana-Sinkam Patrick, Lee L James, Paulaitis Michael E

Abstract excerpt

We present a method that takes advantage of the fluorophore loading dependence of fluorescence nanoparticle tracking (fNTA) to determine the content of specific miRNA targets in extracellular vesicles (EVs) and their stoichiometry across the entire EV population. The method is based on an assay for detecting EV miRNA by hybridization to fluorescently labeled, miRNA-specific molecular beacons encapsulated in...

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PMID: 27989853
DOI: 10.1016/j.nano.2016.10.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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