Article
RNA-templated single-base mutation detection based on T4 DNA ligase and reverse molecular beacon.
Talanta - 15 Jun 2008
Tang Hongxing, Yang Xiaohai, Wang Kemin, Tan Weihong, Li Huimin, He Lifang, Liu Bin
Abstract excerpt
A novel RNA-templated single-base mutation detection method based on T4 DNA ligase and reverse molecular beacon (rMB) has been developed and successfully applied to identification of single-base mutation in codon 273 of the p53 gene. The discrimination was carried out using allele-specific primers, which flanked the variable position in the target RNA and was ligated using T4 DNA ligase only when the primers...
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