Article
A gel-free SNP genotyping method: bioluminometric assay coupled with modified primer extension reactions (BAMPER) directly from double-stranded PCR products.
Human mutation - 1 Aug 2004
Zhou Guo-Hua, Shirakura Hiromi, Kamahori Masao, Okano Kazunori, Nagai Keiichi, Kambara Hideki
Abstract excerpt
Inexpensive, high-throughput genotyping methods are needed for analyzing human genetic variations. We have successfully applied the regular bioluminometric assay coupled with modified primer extension reactions (BAMPER) method to single-nucleotide polymorphism (SNP) typing as well as the allele frequency determination for various SNPs. This method includes the production of single-strand target DNA from a genome...
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