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Article

Comparison of the quantification of KRAS mutations by digital PCR and E-ice-COLD-PCR in circulating-cell-free DNA from metastatic colorectal cancer patients.

Clinica chimica acta; international journal of clinical chemistry - 1 Feb 2017

Sefrioui David, Mauger Florence, Leclere Laurence, Beaussire Ludivine, Di Fiore Frédéric, Deleuze Jean-François, Sarafan-Vasseur Nasrin, Tost Jörg

Abstract excerpt

Circulating cell-free DNA (ccfDNA) bears great promise as biomarker for personalized medicine, but ccfDNA is present only at low levels in the plasma or serum of cancer patients. E-ice-COLD-PCR is a recently developed enrichment method to detect and identify mutations present at low-abundance in clinical samples. However, recent studies have shown the importance to accurately quantify low-abundance mutations as...

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Identifiers

PMID: 27940131
DOI: 10.1016/j.cca.2016.12.004

Genes

KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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