Article
Ultrasensitive detection and identification of BRAF V600 mutations in fresh frozen, FFPE, and plasma samples of melanoma patients by E-ice-COLD-PCR.
Analytical and bioanalytical chemistry - 1 Sept 2014
How-Kit Alexandre, Lebbé Céleste, Bousard Aurélie, Daunay Antoine, Mazaleyrat Nicolas, Daviaud Christian, Mourah Samia, Tost Jörg
Abstract excerpt
A number of molecular diagnostic methods have been developed for the detection and identification of mutations in tumor samples, which are important for the choice of treatment in the context of personalized medicine. For the treatment of metastatic melanoma, Vemurafenib is recommended for patien...
Topics
- Cell Line, Tumor
- Codon
- DNA Mutational Analysis
- DNA Primers
- Genes, ras
- Genotype
- Humans
- Indoles
- Melanoma
- Mutation
- Polymerase Chain Reaction
- Proto-Oncogene Proteins B-raf
- Reproducibility of Results
- Sulfonamides
- Vemurafenib