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An improved digital polymerase chain reaction protocol to capture low-copy KRAS mutations in plasma cell-free DNA by resolving 'subsampling' issues.

Molecular oncology - 1 Oct 2017

Ono Yusuke, Sugitani Ayumu, Karasaki Hidenori, Ogata Munehiko, Nozaki Reo, Sasajima Junpei, Yokochi Tomoki, Asahara Shingo, Koizumi Kazuya, Ando Kiyohiro, Hironaka Katsunori, Daito Tsutomu, Mizukami Yusuke

Abstract excerpt

Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell-free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the major technical challenge for the widespread implementation of cfDNA genotyping as a diagnostic tool is the insufficient sensitivity and specificity of detecting early-stage...

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Identifiers

PMID: 28691390
DOI: 10.1002/1878-0261.12110
PMCID: PMC5623814

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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