Article
Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 2016
Jiang Xin, Smirnova Irina, Kasho Vladimir, Wu Jianping, Hirata Kunio, Ke Meng, Pardon Els, Steyaert Jan, Yan Nieng, Kaback H Ronald
Abstract excerpt
The lactose permease of Escherichia coli (LacY), a dynamic polytopic membrane protein, catalyzes galactoside-H+ symport and operates by an alternating access mechanism that exhibits multiple conformations, the distribution of which is altered by sugar binding. We have developed single-domain camelid nanobodies (Nbs) against a mutant in an outward (periplasmic)-open conformation to stabilize this state of the...
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