Article
Crystal Structure of a ligand-bound LacY-Nanobody Complex.
Proceedings of the National Academy of Sciences of the United States of America - 28 Aug 2018
Kumar Hemant, Finer-Moore Janet S, Jiang Xiaoxu, Smirnova Irina, Kasho Vladimir, Pardon Els, Steyaert Jan, Kaback H Ronald, Stroud Robert M
Abstract excerpt
The lactose permease of Escherichia coli (LacY), a dynamic polytopic membrane transport protein, catalyzes galactoside/H+ symport and operates by an alternating access mechanism that exhibits multiple conformations, the distribution of which is altered by sugar-binding. Camelid nanobodies were made against a double-mutant Gly46 → Trp/Gly262 → Trp (LacYWW) that produces an outward-open conformation, as opposed to...
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