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Article

Evidence for an intermediate conformational state of LacY.

Proceedings of the National Academy of Sciences of the United States of America - 20 Mar 2012

Jiang Xiaoxu, Guan Lan, Zhou Yonggang, Hong Wen-Xu, Zhang Qinghai, Kaback H Ronald

Abstract excerpt

LacY mutant Cys154 → Gly exhibits a periplasmic-closed crystal structure identical to the WT, but is periplasmic-open in the membrane. The mutant hardly catalyzes transport, but binds galactosides from either side of the membrane with the same affinity and is resistant to site-directed proteolysis relative to the pseudo-WT. Site-directed alkylation was also applied to 11 single-Cys mutants in Cys154 → Gly LacY in...

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Identifiers

PMID: 22355148
DOI: 10.1073/pnas.1201107109
PMCID: PMC3311394

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evidence for an intermediate conformational state of LacY.PMID 22355148Read ↗

Residues gating the periplasmic pathway of LacY.Semantic proximity Read ↗

Sugar binding induces an outward facing conformation of LacY.Semantic proximity Read ↗

Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.Semantic proximity Read ↗

An early event in the transport mechanism of LacY protein: interaction between helices V and I.Semantic proximity Read ↗

Crystal Structure of a ligand-bound LacY-Nanobody Complex.Semantic proximity Read ↗

Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport.Semantic proximity Read ↗

Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins.Semantic proximity Read ↗

Single-molecule FRET reveals sugar-induced conformational dynamics in LacY.Semantic proximity Read ↗

YidC protein, a molecular chaperone for LacY protein folding via the SecYEG protein machinery.Semantic proximity Read ↗

Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane.Semantic proximity Read ↗

Outward-facing conformers of LacY stabilized by nanobodies.Semantic proximity Read ↗

Structure of a stabilizing disulfide bridge mutant that closes the active-site cleft of T4 lysozyme.Semantic proximity Read ↗

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