Article
Evidence for an intermediate conformational state of LacY.
Proceedings of the National Academy of Sciences of the United States of America - 20 Mar 2012
Jiang Xiaoxu, Guan Lan, Zhou Yonggang, Hong Wen-Xu, Zhang Qinghai, Kaback H Ronald
Abstract excerpt
LacY mutant Cys154 → Gly exhibits a periplasmic-closed crystal structure identical to the WT, but is periplasmic-open in the membrane. The mutant hardly catalyzes transport, but binds galactosides from either side of the membrane with the same affinity and is resistant to site-directed proteolysis relative to the pseudo-WT. Site-directed alkylation was also applied to 11 single-Cys mutants in Cys154 → Gly LacY in...
Topics
- Carbohydrates
- Cell Membrane
- Crystallography, X-Ray
- Cysteine
- Cytoplasm
- Detergents
- Dose-Response Relationship, Drug
- Escherichia coli
- Escherichia coli Proteins
- Ligands
- Maltose
- Membrane Transport Proteins
- Monosaccharide Transport Proteins