Article
Discovery of rice essential genes by characterizing a CRISPR-edited mutation of closely related rice MAP kinase genes.
The Plant journal : for cell and molecular biology - 1 Feb 2017
Minkenberg Bastian, Xie Kabin, Yang Yinong
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system depends on a guide RNA (gRNA) to specify its target. By efficiently co-expressing multiple gRNAs that target different genomic sites, the polycistronic tRNA-gRNA gene (PTG) strategy enables multiplex gene editing in the family of closely related mitogen-activated protein kinase (MPK) genes in...
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