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Serial sequencing of isolength RAD tags for cost-efficient genome-wide profiling of genetic and epigenetic variations.

Nature protocols - 1 Nov 2016

Wang Shi, Liu Pingping, Lv Jia, Li Yangping, Cheng Taoran, Zhang Lingling, Xia Yu, Sun Hongzhen, Hu Xiaoli, Bao Zhenmin

Abstract excerpt

Isolength restriction site-associated DNA (isoRAD) sequencing is a very simple but powerful approach that was originally developed for genome-wide genotyping at minimal labor and cost, and it has recently extended its applicability to allow quantification of DNA methylation levels. The isoRAD method is distinct from other genotyping-by-sequencing (GBS) methods because of its use of special restriction enzymes to...

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PMID: 27711051
DOI: 10.1038/nprot.2016.133

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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