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Article

A procedure for highly specific, sensitive, and unbiased whole-genome amplification.

Proceedings of the National Academy of Sciences of the United States of America - 7 Oct 2008

Pan Xinghua, Urban Alexander Eckehart, Palejev Dean, Schulz Vincent, Grubert Fabian, Hu Yiping, Snyder Michael, Weissman Sherman M

Abstract excerpt

Highly specific amplification of complex DNA pools without bias or template-independent products (TIPs) remains a challenge. We have developed a method using phi29 DNA polymerase and trehalose and optimized control of amplification to create micrograms of specific amplicons without TIPs from down to subfemtograms of DNA. With an input of as little as 0.5-2.5 ng of human gDNA or a few cells, the product could be...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 18832167
DOI: 10.1073/pnas.0808028105
PMCID: PMC2563063

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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