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Article

[Construction of bromodomain-deleted BRD7 mutation vector based on homologous recombination and reverse PCR amplification].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences - 28 Sept 2016

Niu Weihong, Wang Xinye, Zhou Yao, Li Xiayu, Li Guiyuan, Zhou Ming

Abstract excerpt

OBJECTIVE: To construct a eukaryotic expression vector of bromodomain-containing protein 7 (BRD7) with deletion of bromodomain (BRD7△brd) using the homologous recombination and reverse PCR amplification techniques. METHODS: The linear DNA fragments of bromodomain-deleted mutation of BRD7 (pIRES2-EGFP- 3Flag/BRD7△brd) were amplified by one pair of reverse PCR primers using high-fidelity enzyme, and then these...

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Share to Chromosomal Proteins, Non-Histone
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 27640804
DOI: 10.11817/j.issn.1672-7347.2016.09.001

Genes

BRD7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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