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Article

A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.

Journal of biomolecular NMR - 1 Oct 2016

Hikone Yuya, Hirai Go, Mishima Masaki, Inomata Kohsuke, Ikeya Teppei, Arai Souichiro, Shirakawa Masahiro, Sodeoka Mikiko, Ito Yutaka

Abstract excerpt

Structural analyses of proteins under macromolecular crowding inside human cultured cells by in-cell NMR spectroscopy are crucial not only for explicit understanding of their cellular functions but also for applications in medical and pharmaceutical sciences. In-cell NMR experiments using human cultured cells however suffer from low sensitivity, thus pseudocontact shifts from protein-tagged paramagnetic...

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PMID: 27631409
DOI: 10.1007/s10858-016-0059-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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