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DOTA-amide lanthanide tag for reliable generation of pseudocontact shifts in protein NMR spectra.

Bioconjugate chemistry - 19 Oct 2011

Graham Bim, Loh Choy Theng, Swarbrick James David, Ung Phuc, Shin James, Yagi Hiromasa, Jia Xinying, Chhabra Sandeep, Barlow Nicholas, Pintacuda Guido, Huber Thomas, Otting Gottfried

Abstract excerpt

Structural studies of proteins and protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy can be greatly enhanced by site-specific attachment of lanthanide ions to create paramagnetic centers. In particular, pseudocontact shifts (PCS) generated by paramagnetic lanthanides contain important and unique long-range structure information. Here, we present a high-affinity lanthanide binding tag that...

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PMID: 21877751
DOI: 10.1021/bc200353c

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