Article
Transcription of telomeric DNA leads to high levels of homologous recombination and t-loops.
Nucleic acids research - 2 Nov 2016
Kar Anirban, Willcox Smaranda, Griffith Jack D
Abstract excerpt
The formation of DNA loops at chromosome ends (t-loops) and the transcription of telomeres producing G-rich RNA (TERRA) represent two central features of telomeres. To explore a possible link between them we employed artificial human telomeres containing long arrays of TTAGGG repeats flanked by the T7 or T3 promoters. Transcription of these DNAs generates a high frequency of t-loops within individual molecules...
Topics
- Homologous Recombination
- Humans
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- Telomere
- Telomeric Repeat Binding Protein 1
- Telomeric Repeat Binding Protein 2
- Transcription, Genetic