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Article

Morphing activity between structurally similar enzymes: from heme-free bromoperoxidase to lipase.

Biochemistry - 8 Dec 2009

Chen Bo, Cai Zhen, Wu Wei, Huang Yunlong, Pleiss Juergen, Lin Zhanglin

Abstract excerpt

In this study, to explore the plasticity of the alpha/beta-hydrolase fold family, we converted bromoperoxidase A2 (BPO-A2) from Streptomyces aureofaciens to a lipase by structure comparison with lipase A (LipA) from Bacillus subtilis. These two enzymes have similar structures (2.1 A rmsd) and a very low level of sequence identity ( approximately 18%). A variant BL1 was constructed by deleting the caplike domain...

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Identifiers

PMID: 19883129
DOI: 10.1021/bi9014727

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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