[Application of CRISPR/Cas9 Gene Editing Technique to Establish S1PR5 Gene Knockout Mice].

doi:10.7534/j.issn.1009-2137.2016.04.036

Article

[Application of CRISPR/Cas9 Gene Editing Technique to Establish S1PR5 Gene Knockout Mice].

Zhongguo shi yan xue ye xue za zhi - 1 Aug 2016

Gu Zhen-Yang, Zhao Xiao-Li, Yang Nan, Wang Li, Wang Fei-Yan, Wang Li-Li, Gao Chun-Ji

Abstract excerpt

OBJECTIVE: To establish the S1PR5 gene knockout mouse model by using CRISPR/Cas9 gene editing technique so as to provide the tool for studying the regulating role of sphingosine-1-phosphate receptor 5 (S1PR5) in allogeneic hematopoietic stem cell transplantation. METHODS: Single guide RNA (sgRNA) plasmids against the exon 3 of S1PR5 were designed and constructed. Then the sgRNA and hCas9 were transcribed by T7...

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[Application of CRISPR/Cas9 Gene Editing Technique to Establish S1PR5 Gene Knockout Mice].

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