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Article

Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology.

Journal of immunological methods - 1 Aug 2021

Dong Yan, Bai Haitao, Dong Fang, Zhang Xiao-Bing, Ema Hideo

Abstract excerpt

The CRISPR/Cas9 system has been used for genome editing of human and mouse cells. In this study, we established a protocol for gene knockout (KO) in mouse hematopoietic stem cells (HSCs). HSCs were highly purified from the bone marrow of tamoxifen-treated Cas9-EGFP/Cre-ER transgenic mice, maintained in serum-free polyvinyl alcohol culture with cytokines, lentivirally transduced with sgRNA-Crimson, and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 33957108
DOI: 10.1016/j.jim.2021.113070

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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