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Article

Improvement in the thermostability of chitosanase from Bacillus ehimensis by introducing artificial disulfide bonds.

Biotechnology letters - 1 Oct 2016

Sheng Jun, Ji Xiaofeng, Zheng Yuan, Wang Zhipeng, Sun Mi

Abstract excerpt

OBJECTIVE: To determine the effects of artificial disulfide bridges on the thermostability and catalytic efficiency of chitosanase EAG1. RESULTS: Five artificial disulfide bridges were designed based on the structural information derived from the three-dimensional (3-D) model of chitosanase EAG1. Two beneficial mutants (G113C/D116C, A207C-L286C) were located in the flexible surface loop region, whereas the...

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PMID: 27395063
DOI: 10.1007/s10529-016-2168-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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