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Directed evolution to enhance secretion efficiency and thermostability of chitosanase from Mitsuaria chitosanitabida 3001.

Bioscience, biotechnology, and biochemistry - 1 Feb 2006

Yun Choongsoo, Matsuda Hideyuki, Kawamukai Makoto

Abstract excerpt

Chitosanase (ChoA) from Mitsuaria chitosanitabida 3001 was successfully evolved with secretion efficiency and thermal stability. The inactive ChoA mutant (G151D) gene was used to mutate by an error-prone PCR technique and mutant genes that restored chitosanase activity were isolated. Two desirable mutants, designated M5S and M7T, were isolated. Two amino acids, Leu74 and Val75, in the signal peptide of ChoA were...

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Burkholderia
Chitosan
Directed Molecular Evolution
Enzyme Stability
Glycoside Hydrolases

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PMID: 16495682
DOI: 10.1271/bbb.70.559

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