Article
The hydrogen-bond network around Glu160 contributes to the structural stability of chitosanase CsnA from Renibacterium sp. QD1.
International journal of biological macromolecules - 1 Apr 2018
Han Yujuan, Yu Rilei, Gao Peixin, Lu Xinzhi, Yu Wengong
Abstract excerpt
CsnA, a chitosanase from Renibacterium sp. QD1, has great potential for industrial applications due to its high yield and broad pH stability. In this study, a specific Glu160 in CsnA was identified by sequence alignment, and structural analysis and MD simulation predicted that Glu160 formed a hydrogen-bond network with Lys163 and Thr114. To evaluate the effect of the network, we constructed four mutants,...
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