Article
Lateral flow strip for visual detection of K-ras mutations based on allele-specific PCR.
Biotechnology letters - 1 Oct 2016
Wang Cong, Chen Xiaomin, Wu Yuying, Li Hao, Wang Yu, Pan Xiaofu, Tang Tingting, Liu Ziying, Li Xiaokun
Abstract excerpt
OBJECTIVES: To develop a convenient and sensitive point-of-care test for detecting gene mutations based on allele-specific PCR. RESULTS: To develop a lateral flow strip for visual detection of K-ras mutations based on a modified PCR, a specific DNA tag was covalently linked to the 5'-end of each primer by a nine-carbon linker to produce a sticky end. One of the sticky ends of the PCR products bound to gold...
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